Cryopreservation

Cryopreservation​ clinic in Bangalore

Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. -196 Celsius.  The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful Cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.

Cryopreservation Techniques

There are conditions like cancer, where preserving fertility becomes essential. In such a situation, couples can have biological children when their health is restored. The advancement in technology has enabled the preservation of fertility using several methods. Cryopreservation procedure is one such method of sperm or egg freezing and even embryo freezing.

  • Fertility preservation techniques give women and men more flexibility when it comes to family planning.
  • Cryopreservation techniques involve is basically freezing the sperms, eggs, or embryos at a very low temperature (sub-zero) in compounds like liquid nitrogen. ( -196 Celsius) The low temperatures allow cells to have indefinite longevity by suspending all biological activity in them. When the sperms or embryos are needed, they are fertilized or used in a treatment cycle.

Sperm Freezing

  • A man can preserve his sperms for having children in the future with IVF and other such techniques. A man is required to provide a sample for preservation. If no sperms are present, a surgical sperm extraction can be performed to remove sperms directly from the testicles. These are stored in the bank until ready to be used for different fertility procedures

Egg Freezing

  • Fertility declines in women rapidly after the age of 35. Egg freezing is an ideal choice in such cases. Women, when they are young, can freeze their eggs in the laboratories to have children at a later age.
  • Females who have to undergo chemotherapy can also freeze their eggs before receiving the treatment.

Embryo Freezing

  • In the IVF process, the eggs are fertilized with the partner’s sperm. The resulting embryos are cultured, allowing the cell division to reach the optimal level. When the embryos are formed, they are stored at a fertility clinic until they are to be transferred.
Get in touch

Getting a customized treatment can be one of best experiences that you can have.

Testimonials

Aansh Ivf Hospital